Gramolini Lab

Select Publications

REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects

SH Lee, Hadipour-Lakmehsari S, Murthy HR, Gibb N, Miyake T, Teng ACT, Cosme J, Yu JC, Moon M, Lim S, Wong V, Liu P, Billia F, Fernandez-Gonzalez R, Stagljar I, Sharma P, Kislinger T, Scott IC, Gramolini AO. (2020) Nature Communications. 2020 Feb 19;11(1):965. doi: 10.1038/s41467-019-14143-9.

Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium

SH Lee, Hadipour-Lakmehsari S, Kim DH, Di Paola M, Kuzmanov U, Shah S, Lee JJ, Kislinger T, Sharma P, Oudit GY, Gramolini AO. (2020) Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium. Scientific Data. 2020 Dec 1;7(1):425. doi: 10.1038/s41597-020-00762-1.

Mapping signalling perturbations in myocardial fibrosis via the integrative phosphoproteomic profiling of tissue from diverse sources

Kuzmanov U, Wang EY, Vanderlaan R, Kim DH, Lee SH, Hadipour-Lakmehsari S, Guo H, Zhao Y, McFadden M, Sharma P, Billia F, Radisic M, Gramolini A*, Emili A*. (2020) Mapping signalling perturbations in myocardial fibrosis via the integrative phosphoproteomic profiling of tissue from diverse sources. Nature Biomedical Engineering. 2020 Sep;4(9):889-900. doi: 10.1038/s41551-020-0585-y. Epub 2020 Jul 13.

Nanoscale reorganization of sarcoplasmic reticulum in pressure-overload cardiac hypertrophy visualized by dSTORM

Hadipour-Lakmehsari S, Driouchi A, Lee SH, Kuzmanov U, Callaghan NI, Heximer SP, Simmons CA, Yip CM, Gramolini AO. (2019) Nanoscale reorganization of sarcoplasmic reticulum in pressure-overload cardiac hypertrophy visualized by dSTORM. Scientific Reports. 2019 May 27;9(1):7867. doi: 10.1038/s41598-019-44331-y.

Global phosphoproteomic profiling reveals perturbed signaling in a mouse model of dilated cardiomyopathy

U Kuzmanov, H Guo, J Cosme, R Isserlin, P Sharma, A.O Gramolini*, A Emili*. (2016). Global phosphoproteomic profiling reveals perturbed signaling in a mouse model of dilated cardiomyopathy. Proc Natl Acad Sci (USA)113(44):12592-12597

Self-renewing resident arterial macrophages arise from embryonic CX3CR1⁺ precursors and circulating monocytes immediately after birth

Ensan S, Li A, Besla R, Degousee N, Cosme J, Roufaiel M, Shikatani EA, El-Maklizi M, Williams JW, Robins L, Li C, Lewis B, Yun TJ, Lee JS, Wieghofer P, Khattar R, Farrokhi K, Byrne J, Ouzounian M, Zavitz CC, Levy GA, Bauer CM, Libby P, Husain M, Swirski FK, Cheong C, Prinz M, Hilgendorf I, Randolph GJ, Epelman S, Gramolini AO, Cybulsky MI, Rubin BB, Robbins CS. (2016). Self-renewing resident arterial macrophages arise from embryonic CX3CR1(+) precursors and circulating monocytes immediately after birth. Nature Immunology.17(2): 159-68.

Targeted proteomics identifies liquid-biopsy signatures for extracapsular prostate cancer

Kim Y, Jeon J, Mejia S, Yao CQ, Ignatchenko V, Julius O, Nyalwidhe N, Gramolini AO, Lance RS,Troyer DA, Drake RR, Boutros PC, Semmes OJ and Kislinger T. (2016). Targeted proteomics identifies liquid biopsy signatures for extracapsular prostate cancer. Nature Communications.7: 11906.

Identifying Low-Abundance Biomarkers Aptamer-Based Proteomics Potentially Enables More Sensitive Detection in Cardiovascular Diseases

Gramolini A, Lau E, Liu PP.(2016). Identifying Low-Abundance Biomarkers: Aptamer-Based Proteomics Potentially Enables More Sensitive Detection in Cardiovascular Diseases. Circulation. 116: 116.022940.

Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function

Sharma P, Abbasi C, Bousette N, Lazic S, Dubois N, Ignatchenko A, Ignatchenko V, Teng A, Wilson A, Noronha M, Wong V, Liu J, Araki T, Liu J, Tiburcy M, Zimmerman WH, Ackerley C, Hamilton R, Sun Y, Liu P, Backx P, Keller G, Stagljar I, Scott I, Kislinger T, Gramolini AO. Tmem65 is an Evolutionary Conserved Membrane Protein that Regulates Cardiac GAP Junction communication. Nature Communications. 2015; 10.1038/NCOMMS9391.

Metformin Increases Degradation of Ubiquitinylated Phospholamban via Autophagy in Cardiomyocytes

Teng A, Miyake T, Yokoe S, Zhang, L, Rezende ML, Maclennan DH, Liu PP, Gramolini AO. Metformin Increases Degradation of Ubiquitinylated Phospholamban via Autophagy in Cardiomyocytes. Proc Natl Acad Sci (USA). 2015;112:7165-7170.

Systems analysis reveals down-regulation of a network of pro-survival miRNAs drives the apoptotic response in dilated cardiomyopathy

Isserlin R, Merico D, Wang D, Bousette N, Gramolini AO, Bader G, Emili A. Systems analysis reveals down-regulation of a network of pro-survival miRNAs drives the apoptotic response in dilated cardiomyopathy. Molecular BioSystems. 2015;11:239-251.

Endoplasmic Reticulum Resident Protein 44 (ERp44) Deficiency in Mice and Zebrafish Leads to Cardiac Developmental and Functional Defects

Wang D, Abbasi C, El-Rass S, Li J, Dawood F, Naito K, Sharma P, Bousette N, Singh S, Backx PH, Cox B, Wen XY, Liu P, Gramolini AO. ERp44 deficiency in mice and zebrafish leads to cardiac developmental and functional defects. Journal of American Heart Association. 2014;3:e001018.

HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress

Zhang L, Chen X, Sharma P, Moon M, Sheftel AD, Dawood F, Nghiem MP, Wu J, Li RK, Gramolini AO, Sorensen PH, Penninger JM, Brumell JH, Liu PP. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nature Communications. 2014;5:3430.

Cathepsin‐L Ameliorates Cardiac Hypertrophy Through Activation of the Autophagy–Lysosomal Dependent Protein Processing Pathways

Sun M, Ouzounian M, de Couto G, Chen M, Yan R, Fukuoka M, Li G, Moon M, Liu Y, Gramolini A, Wells GJ, Liu PP. Cathepsin-L ameliorates cardiac hypertrophy through activation of the autophagy-lysosomal dependent protein processing pathways. J Am Heart Assoc. 2013 Apr 22;2(2):e000191. doi: 10.1161/JAHA.113.000191

Structural determination of the phosphorylation domain of the ryanodine receptor

P. Sharma, U.Nair, W. Li, A. Dong, T. Miyake, A. Wilson, T.Ryan, T. Kislinger, S.Dhe-Paganon, and A.O.Gramolini (2012) Structural Determination of the Phosphorylation Domain of the Ryanodine Receptor. Febs J 279, 3952-3964.

P.J. Rugg-Gunn, B.J. Cox, F. Lanner, P. Sharma, V. Ignatchenko, A.C. McDonald, J. Garner, A.O. Gramolini, J. Rossant, T. Kislinger (2012) Cell-Surface Proteomics Identifies Lineage-Specific Markers of Embryo-Derived Stem Cells. Developmental Cell 22, 887-901.

SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells

N. DuBois, A. Craft, P. Sharma, A. Elephanty, E. Stanley, D. Elliott, A. Gramolini, and G. Keller (2011) Novel use for SIRPA as a specific cell surface marker for the isolation of human pluripotent stem cell-derived cardiomyocytes. Nature Biotechnology 29, 1011-1018.